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KMID : 1094720000050040227
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 4 p.227 ~ p.233
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Characterization of the α-mannosidase gene family in filamentous fungi: N-glycan remodelling for the development of eukaryotic expression systems
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Eades C. Joshua
Hintz William E.
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Abstract
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Although filamentous fungi are used extensively for protein expression, their use for the production of heterologous glycoproteins is constrained by the types of N-glycan structures produced by filamentous fungi as compared to those naturally found on the glycoproteins. Attempts are underway to engineer the N-glycan synthetic pathways in filamentous fungi in order to produce fungal expression strains which can produce heterologous glycoproteins carrying specific N-glycan structures. To fully realize this goal, a detailed understanding of the genetic components of this pathway in filamentous fungi is required. In this review, we discuss the characterization of the α-mannosidase gene family in filamentous fungi and its implications for the elucidation of the N-glycan synthetic pathway.
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KEYWORD
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glycosylation, filamentous fungi, N-glycans, genetic manipulations
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